Nicholas G. Theodorakis, Ph.D. Molecular Biologist

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Work Experience

• September 2010–present: Technical Support Consultant. Roche Diagnostics Corp., Roche Applied Sciences (through Adecco), Indianapolis, IN
  • Providing technical support for Roche Applied Sciences research products and equipment.

Faculty Appointments

• March 2010–present: Adjunct Professor. Ivy Tech Community College, Dept. of Workforce and Economic Development, Indianapolis, IN
  • Teaching the course "Biotech/Bioprocess Manufacturing," a non-credit, 160 hour course offered to displaced workers
• August 2004–July 2009: Research Assistant Professor. Indiana University Medical School, Dept. of Surgery, Indianapolis, IN
  • Research into the mechanisms of diseases of the liver and circulatory system using in vitro and small animal models.
• August 1999–July 2004: Assistant Professor. University of Rochester Medical School, Dept. of Surgery, Rochester, NY
  • Research into the mechanisms of diseases of the liver and circulatory system using in vitro and small animal models.
  • Medical student teaching:
    • Tutor for medical student problem-based learning course “From Molecules to Cells.”
    • Organizing and lecturing in a new course for first-year MD-PhD students on “Techniques in Modern Molecular Biology”.
  • Member of University of Rochester Radiation Safety Committee
• December 1998–July 1999: Research Assistant Professor. Georgetown University Medical Center, Dept. of Surgery, Washington, DC
  • Research into the mechanisms of diseases of the liver and circulatory system using in vitro and small animal models.

Post-doctoral training

• May 1989–November 1998: Post-doctoral fellow. Johns Hopkins University Medical Center, Baltimore, MD
  • Research into the mechanisms of post-transcriptional regulation of gene expression using in vitro models.

Skills

Technical

• Bench research using biochemical, molecular biological, and cell biological techniques to examine gene expression and biological markers using in vitro and small animal systems. Specific techniques used include (but not limited to):
  • Cell culture of primary and established cell lines; isolation of primary cells; transfection of cells with plasmids or siRNA, immunofluorescence microscopy
  • Isolation of RNA from cells and tissues; analysis of RNA by RT-PCR, RNase protection, Nuclease S1 protection, or Northern blot
  • Examination of transcriptional activity by nuclear run-off analysis, electrophoretic mobility shift assay (EMSA), or transfection of reporter genes
  • Analysis of proteins by one or two-dimensional SDS-PAGE; Western blotting, enzymatic assays, ELISA, zymography, chromatography, and binding studies
  • Construction and isolation of plasmid vectors used for gene expression studies or probes

Communicative

• Preparation and submission of manuscripts for publication in scientific journals
• Presentation of data in scientific conferences and local informal settings
• Teaching medical students and undergraduates
• Maintaining collaborations with researchers within the same department as well as with those in other universities

Education

• Ph.D. May 1989. Northwestern University, Evanston, IL. Dept. of Biochemistry and Molecular and Cellular Biology.
  Dissertation title: “Post-transcriptional Regulation of HSP70 Expression in Erythroid and Non-erythroid Cells.”
  Thesis advisor: Richard Morimoto, Ph.D.
• B.A. May 1982. Washington University in St. Louis. St. Louis, MO. Major in Biology.

Publications

Complete list of publications (co-author of more than 25 original research papers and book chapters) is available on my publications page.

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